Synucleozid hydrochloride

CAS No. 2741856-68-6

Synucleozid hydrochloride( —— )

Catalog No. M33133 CAS No. 2741856-68-6

Synucleozid hydrochloride inhibits the translation of the intrinsically disordered protein α-synuclein by targeting its structured mRNA.

Purity : >98% (HPLC)

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Biological Information

  • Product Name
    Synucleozid hydrochloride
  • Note
    Research use only, not for human use.
  • Brief Description
    Synucleozid hydrochloride inhibits the translation of the intrinsically disordered protein α-synuclein by targeting its structured mRNA.
  • Description
    Synucleozid hydrochloride (NSC 377363 hydrochloride) is a potent inhibitor of the?SNCA?mRNA?that encodes α-synuclein protein. Synucleozid selectively?targets the?α-synuclein?mRNA 5′ UTR at the designed IRE site, decreases the amount of?SNCA?mRNA loaded into polysomes and thereby inhibits?SNCA?translation. Synucleozid has the potential for the investigation of Parkinson’s disease.
  • In Vitro
    Synucleozid (0.25-1 μM; 24 hours) abrogates cytotoxicity induced by α-synuclein preformed fibrils, which act as seeds and recruit endogenous α-synuclein to aggregate.Synucleozid (0-1 μM; 24 hours) binds to the A bulge near the base of the IRE hairpin, reduced levels of α-synuclein in a dose-dependent manner with an IC50 of 500 nM, and inhibits α-synuclein protein expression in SH-SY5Y neuroblastoma cells.Synucleozid (100 nM-100 μM; 24 hours) binds to 2-AP-labeled and native IRE RNA with similar affinities. It decreases 2-AP emission with an EC50?value of 2.7 ± 0.4 μM, recovery of 2-AP emissions is observed as a function of unlabeled?SNCA?IRE RNA (RNA-0) concentration, affording a competitive?Kd?of 1.5 ± 0.3 μM.Synucleozid (0.25-1 μM; 24 hours) decreases α-synuclein and other proteins that have IREs in their mRNA’s UTR including APP, PrP, Ferritin and TfR as a dose-dependent mannner. All panels is completed in SH-SY5Y cells, except for PrP protein which is assessed in Neuro-2A cells.Cell Viability Assay Cell Line:SH-SY5Y neuroblastoma cells Concentration:0.25 μM, 0.5 μM, 1 μM Incubation Time:24 hours Result:Decreased LDH release as a dose-dependent manner.Western Blot Analysis Cell Line:SH-SY5Y neuroblastoma cells Concentration:0.25 μM, 0.5 μM, 1 μM Incubation Time:24 hours Result:Decreased α-synuclein expression as a concentration-dependent manner.
  • In Vivo
    ——
  • Synonyms
    ——
  • Pathway
    Cell Cycle/DNA Damage
  • Target
    DNA/RNA Synthesis
  • Recptor
    DNA/RNA Synthesis
  • Research Area
    ——
  • Indication
    ——

Chemical Information

  • CAS Number
    2741856-68-6
  • Formula Weight
    404.9
  • Molecular Formula
    C22H21ClN6
  • Purity
    >98% (HPLC)
  • Solubility
    In Vitro:?DMSO : 125 mg/mL (308.72 mM; Ultrasonic )H2O : 2 mg/mL (4.94 mM; Ultrasonic)
  • SMILES
    Cl.N=C(N)C1=CC=C(C=C1)NC=2C=CC(=CC2)C3=CC4=CC=C(C=C4N3)C(=N)N
  • Chemical Name
    ——

Shipping & Storage Information

  • Storage
    (-20℃)
  • Shipping
    With Ice Pack
  • Stability
    ≥ 2 years

Reference

1. Zhang P, et al. Translation of the intrinsically disordered protein α-synuclein is inhibited by a small molecule targeting its structured mRNA.Proc Natl Acad Sci U S A. 2020 Jan 21;117(3):1457-1467.?
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