Synucleozid hydrochloride

Research use only, not for human use.

Synucleozid hydrochloride inhibits the translation of the intrinsically disordered protein α-synuclein by targeting its structured mRNA.

Synucleozid hydrochloride (NSC 377363 hydrochloride) is a potent inhibitor of the?SNCA?mRNA?that encodes α-synuclein protein. Synucleozid selectively?targets the?α-synuclein?mRNA 5′ UTR at the designed IRE site, decreases the amount of?SNCA?mRNA loaded into polysomes and thereby inhibits?SNCA?translation. Synucleozid has the potential for the investigation of Parkinson’s disease.

Synucleozid (0.25-1 μM; 24 hours) abrogates cytotoxicity induced by α-synuclein preformed fibrils, which act as seeds and recruit endogenous α-synuclein to aggregate.Synucleozid (0-1 μM; 24 hours) binds to the A bulge near the base of the IRE hairpin, reduced levels of α-synuclein in a dose-dependent manner with an IC50 of 500 nM, and inhibits α-synuclein protein expression in SH-SY5Y neuroblastoma cells.Synucleozid (100 nM-100 μM; 24 hours) binds to 2-AP-labeled and native IRE RNA with similar affinities. It decreases 2-AP emission with an EC50?value of 2.7 ± 0.4 μM, recovery of 2-AP emissions is observed as a function of unlabeled?SNCA?IRE RNA (RNA-0) concentration, affording a competitive?Kd?of 1.5 ± 0.3 μM.Synucleozid (0.25-1 μM; 24 hours) decreases α-synuclein and other proteins that have IREs in their mRNA’s UTR including APP, PrP, Ferritin and TfR as a dose-dependent mannner. All panels is completed in SH-SY5Y cells, except for PrP protein which is assessed in Neuro-2A cells.Cell Viability Assay Cell Line:SH-SY5Y neuroblastoma cells Concentration:0.25 μM, 0.5 μM, 1 μM Incubation Time:24 hours Result:Decreased LDH release as a dose-dependent manner.Western Blot Analysis Cell Line:SH-SY5Y neuroblastoma cells Concentration:0.25 μM, 0.5 μM, 1 μM Incubation Time:24 hours Result:Decreased α-synuclein expression as a concentration-dependent manner.

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Cell Cycle/DNA Damage

DNA/RNA Synthesis

DNA/RNA Synthesis

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2741856-68-6

404.9

C22H21ClN6

>98% (HPLC)

In Vitro:?DMSO : 125 mg/mL (308.72 mM; Ultrasonic )H2O : 2 mg/mL (4.94 mM; Ultrasonic)

Cl.N=C(N)C1=CC=C(C=C1)NC=2C=CC(=CC2)C3=CC4=CC=C(C=C4N3)C(=N)N

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(-20℃)

With Ice Pack

≥ 2 years